基于Wnt-β-catenin信号通路研究黄精多糖促进骨髓间充质干细胞向成骨

发布于:2021-10-17 02:04:41

目录 本文主要英文缩略词索引 ...................................................................................1 摘要 .......................................................................................................................2 Abstract .................................................................................................................5 前言 ...................................................................................................................8 第一部分 黄精多糖促进骨髓来源的间充质干细胞向成骨细胞分化 .........12 1 材料和方法 .....................................................................................................13 1.1 材料 ...............................................................................................................13 1.1.1 细胞株 .......................................................................................................13 1.1.2 主要仪器设备 ...........................................................................................13 1.1.3 主要耗材 ...................................................................................................14 1.1.4 药物与主要试剂 .......................................................................................15 1.1.5 主要试剂的配制 .......................................................................................16 1.2 方法 ..............................................................................................................16 1.2.1 小鼠间充质干细胞的培养 .......................................................................17 1.2.2 实验分组及细胞接种 ...............................................................................18 1.2.3 MTT 法测定黄精多糖对 BMSCs 细胞增殖的影响 ................................19 1.2.4 碱性磷酸酶活性检测 ...............................................................................19 1.2.5 茜素红染色检测矿化结节 .......................................................................20 1.2.6 实时荧光定量 PCR 检测成骨相关基因..................................................20 万方数据 1.2.7 统计学分析 ...............................................................................................24 2 结果 .................................................................................................................24 2.1 BMSCs 细胞生长情况及形态学变化 .........................................................24 2.2 黄精多糖对细胞增殖的影响 .......................................................................25 2.3 黄精多糖能显著提高 BMSCs 碱性磷酸酶活性 ........................................26 2.4 黄精多糖能明显增强 BMSCs 细胞外基质矿化 ........................................26 2.5 黄精多糖能明

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